University Pathologists - Services - Test Menu

· Routine hematoxylin & eosin histology for surgical specimens, endoscopic biopsies, endoscopic/FNA needle biopsies.

· Cytopathology

- gynecologic and non-gynecologic cytology
- FNA cytology with intraoperative assessment of specimen adequacy

· Hematopathology

- Multidisciplinary approach to hematologic disorders
- Morphologic evaluation of peripheral blood, bone marrow and lymphoid tissue
- Comprehensive panel of immunohistochemical stains
- Comprehensive panel of histochemical stains
- Fully equipped flow cytometry lab with the most recent, comprehensive list of antibodies
- Cytogenetics
- Molecular Pathology

· Special histochemical stains

- Infectious pathogens: 2% Giemsa (routinely performed on upper gastrointestinal endoscopic biopsies), Gram, acid fast bacilli, GMS, PAS, Fite, Warthin Starry, Steiner.
- Tissue stains: PAS w/without diastase, Alcian blue w/without hyaluronidase, Alcian blue/PAS (routinely performed on upper gastrointestinal endoscopic biopsies), Von Kossa, Fontana Masson, Reticulum, Trichrome and Iron (the latter three routinely performed on liver and bone marrow biopsies), Congo red, Oil Red O, PTAH, Van Gieson, Orcein.
- Mucin stains: Mucicarmine, Alcian blue, PAS.

· Immunohistochemical stains

AAT - Alpha-1-Antitrypsin
Actin - Muscle, Myofibroblasts and Myoepithelial Cells
AE1/AE3 - Keratin Cocktail, Low and High Molecular Weight
AFP - Alpha Fetoprotein
ALK-1 - Anaplastic Lymphoma Kinase
B72.3 - Epithelial Marker, Adenocarcinoma
BCL-1 - Mantle Cell Lymphoma (PRAD1)
BCL-2 - Follicular Cell Lymphoma, Inhibitor of Apoptosis
BCL-6 - Follicle Center Cell
BerH2 (CD30) - Activation Marker
BRST-2 - Gross Cystic Disease Fluid Protein
CA 19.9 - Pancreas, Ovary, GI, Lung, Bladder Tumors
CA125 - Ovary, Breast, Other Adenocarcinomas
Calcitonin - Medullary Carcinoma of Thyroid
Caldesmon - Smooth Muscle cells
Calponin - Myoepithelial, Myofibroblastic Cells, Smooth Muscle Cells
Calretinin - Mesothelial Cells, Sex Cord Stromal Cells, Stromal Tumors, Adrenal Cortical Tumors
Cam5.2 - Keratin Low Molecular Weight
CD1a - Cortical Thymocytes, Langerhans Cells
CD3 - pan-T cell Marker
CD5 - pan-T cell Marker
CD10 - Common Acute Lymphoblastic Leukemia Antigen (CALLA)
CD15 - Epithelial, Myeloid and Reed-Sternberg Cells
CD20 - pan-B cell Antigen
CD30 (BerH2) - Ki 1, Activated T and B cells, Embryonal Carcinoma, Reed-Sternberg Cells, Anaplastic Large Cell Lymphoma
CD31 - Endothelium, Plasma Cells
CD34 - Endothelium, Stem Cells, Gastrointestinal Stromal Tumors, Blasts
CD43 - T-cells, Subset of B-cells, Histiocytes, Myeloid Cells
CD45 - Leukocyte Common Antigen
CD68 - Macrophages
CD79a - B-cells
CD99 - Ewing Sarcoma, PNET
CD117 (c-kit) - Myeloid, Mast Cell Marker, Gastrointestinal Stromal Tumors
CD138 - Plasma Cells
CEA (m) - Monoclonal Carcinoembryonic Antigen, Adenocarcinoma
CEA (p) - Polyclonal Carcinoembryonic Antigen, Adenocarcinoma, Hepatocellular Carcinoma
CHR - Chromogranin, Neuroendocrine and Neural Marker
CK7 - Keratin: 54kD Lung, Breast, Gastric, Pancreatic, Billiary, Ovarian Carcinomas
CK20 - Keratin: 46kD Intestinal Adenocarcinomas
CK903 - High Molecular Weight Cytokeratin
CMV - Cytomegalovirus
Desmin - Muscle, Desmoplastic Small Round Cell Tumor
E-cadherin - Epithelial Marker
EGFR - Epidermal Growth Factor Receptor
EMA - Epithelial Membrane Antigen
ER - Estrogen Receptor
Factor VIII - Von Willebrand Factor, Endothelial Cells and Megakaryocytes
Factor XIIIa - Fibrohistiocytic Tumors
GFAP - Glial Fibrillary Acidic Protein, Astrocytic Tumors
HCG - Human Chorionic Gonadotropin
HMB 45 - Melanoma Marker, Angiomyolipoma, PEComas
HepPar1 - Hepatocyte Marker
HPAP - Human Prostate Acid Phosphatase
HPV - Human Papilloma Virus
HSV I+II - Herpes Simplex Virus
Inhibin - Sex Cord Stromal Tumors, Adrenal Cortical Tumors
Kappa - Immunoglobulin Kappa Light Chain
Ki-67 - Proliferation Marker
Lambda - Immunoglobulin Lambda Light Chain
hMLH-1 - MMR, Mismatch Repair Protein
hMSH-2 - MMR, Mismatch Repair Protein
Mart-1 - Melanoma Marker
Melan A - Melanoma Marker, Adrenal Cortical Tumors
MPO - Myeloperoxidase
MYOD1 - Skeletal Muscle Antigen, Rhabdomyosarcoma
NF - Neurofilament, Neural Tumors, Merkel Cell Tumor
p53 - Mutated Tumor Suppressor Gene Product
PIN cocktail - Racemase P504S + p63, Prostatic Intraepithelial Neoplasia vs Invasive Prostatic Adenocarcinoma
PLAP - Placental Alkaline Phosphatase, Germ Cell Tumors, Trophoblastic Disease
PR - Progesterone Receptor
PSA - Prostate Specific Antigen
RCC - Renal Cell Carcinoma Marker
S-100 - S100 Protein, Nerve Sheath Tumor, Melanoma, Chondrocytes
SMA - Smooth Muscle Actin, Myoepithelial and Myofibroblastic Cells
SMMS - Smooth Muscle Myosin
SYN - Synaptophysin, Neural and Neuroendocrine Marker
TdT - Terminal Deoxinucleotidyl Transferase, Cortical Thymocytes, Lymphoblastic Leukemia
THYRO - Thyroglobulin
TTF-1 - Thyroid Transcription Factor - 1, Lung and Thyroid Carcinomas, Non-specific Cytoplasmic Staining of Hepatocytes
ULEX - Ulex Europaeus Agglutinin, Endothelium, Platelets, Collecting Duct Carcinoma
VIM - Vimentin, Cytoplasmic Intermediate Filament
WT-1 - Mesothelial and Mullerian Tumors, Desmoplastic Small Round Cell Tumor, Sex Cord Stromal Tumors

· Direct & Indirect Immunofluorescence

· Flow cytometry

- Our lab is equipped with 4-color analysis Becton-Dickinson FACSCalibur flow cytometer, performing the following tests:
- Leukemia/lymphoma immunophenotyping panels; analysis can be performed on blood, bone marrow, peritoneal and cerebrospinal fluid, solid tissues (lymph nodes, spleen, liver, skin, etc)
- Quantification of CD4+ , CD8+, and CD3+ cells (percentage and absolute count) for AIDS panels
- Quantification of any cell subset (T cells, B cells, myeloid cells)
- Quantification of residual WBC in blood components (platelet concentrates, red blood cell concentrates, etc).
- Detection of cytoplasmic antigens (kappa, lambda, ZAP-70, cytokines, others)
- DNA ploidy and cell cycle analysis
- Apoptosis and cell viability (TUNEL assay, Annexin V, propidium iodide)
- Tetramer analysis to detect T cells responsive to specific antigens (in development).

· Molecular and genetic testing

<< PREVIOUS | HOME